Worm Recovery and Precipitin Antibody Response in Guinea Pigs and Rats Infected with Clonorchis Sinensis
Resource
微免與感染雜誌 v.31 n.4 pp.211-216
Journal
微免與感染雜誌,v.31
Journal Issue
n.4
Pages
211-216
Date Issued
1998
Date
1998
Author(s)
SU, KUA-EYRE
WANG, FU-YIN
CHI, PAO-YU
Abstract
Guinea pigs (Hartley strain) and rats (Wistar strain) were each fed 200 and 100 Clonorchis sinensis metacercariae, respectively. Five animals from each species were sacrificed weekly between 1-8 weeks postinfection (WPI) and then at 12 , 16, 20, and 30 WPI for collection of worms, bile and sera . The overall worm recovery rates for guinea pigs and rats were 18.7% and 12.4%, respectively. Only one of the five rats examined at 20 WPI still harbored one worm, while all were worm-free at 30 WPI. By a double diffusion test, no antibodies were detected against C. sinensis adult antigens in the bile juice. Serum antibodies were detected in at least 95% of the infected guinea pigs between 4-30 WPI and rats between 3-16 WPI. Precipitin antibodies seemed to be correlated with the presence of live worms in rats that had been infected for more than 12 weeks.