Investigation on the potential anti-Alzheimer’s disease plant materials in a transgenic Caenorhabditis elegans
Date Issued
2010
Date
2010
Author(s)
Cheng, Kai-Yuan
Abstract
Alzheimer''s disease (AD) is a progressive neurodegenerative disorder that leads to cognitive, memory and behavioural impairments. AD patients have two neuropathological characteristics in brain: extracellular accumulation of β amyloid peptide (Aβ) and the formation of intracellular neurofibrillary tangles (NTF) of hyperphosphorylated tau protein. Caenorhabditis elegans is a small, free-living soil nematode. It is widely employed in the studies of developmental biology, genetics and neurobiology. The AD transgenic C. elegans CL4176 which carry the human gene for Aβ1-42 have intracellular amyloid formation in body-wall muscle cells, and can develop concomitant rapid paralysis by temperature up-shift. Moreover, CL4176 exhibits increased levels of reactive oxygen species (ROS) and protein carbonyl content, similar to those observed in AD patients. Thus, this transgenic C. elegans CL4176 is a suitable in vivo model for AD research.
The aim of this study is to screen out the potential anti-AD activity compounds, including EGb761, sesamol, tetrahydrocurcumin (THC), gallic acid, n-butanol fraction of 70% methanol extract of embryo of Nelumbo nucifera Gaertn. Seed (Nn-M-B) by AD transgenic C. elegans model CL4176 and to study the possible mechanisms for their efficacy. First, we found that EGb761 100 ppm, sesamol 10 ppm, Nn-M-B V and Nn-M-B VII 100 ppm could delay the paralysis in CL4176 after temperature up-shift. Furthermore, sesamol and Nn-M-B VII increased the expression of Hsp16, Hsp40 and Hsp70. and inhibited Aβ aggregation in CL4176 by Western blot. We also found that EGb761, sesamol could increase the activity of catalase, and reduced the level of ROS in CL4176. These finding suggest that EGb 761, sesamol, Nn-M-B V and Nn-M-B VII delay the paralysis in CL4176, possibly by inducing HSPs expression and inhibiting Aβ aggregation. Furthermore, EGb 761, sesamol increase the activity of catalase and reduce the level of ROS in CL4176.
These results suggest that EGb761, sesamol, Nn-M-B V and Nn-M-B VII have potential for prevention and treatment of AD.
Subjects
β amyloid peptide
AD transgenic C. elegans CL4176
Nelumbo nucifera Gaertn. Seed
SDGs
Type
thesis
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