Effect of Egr1 gene knockdown on ocular development in zebrafish
Date Issued
2004
Date
2004
Author(s)
Hu, Chao-Yu
DOI
zh-TW
Abstract
Purpose: ZENK gene has been proposed to be associated with abnormal eye growth in chickens. The aim of this study is to identify the change in early ocular development after Egr1 (early growth response 1) gene, the homologue of ZENK gene, knockdown in zebrafish by morpholino oligo-nucleotides (MO), which is a sequence-specific anti-sense mRNA inhibitor.
Methods: Various doses of Egr1-MO were microinjected into the 1- to 4-cell zebrafish embryos. In addition, chordin MO and the standard control MO were also injected as positive control and negative control respectively. After correction with the death rate at 24 hours post-fertilization (hpf) and abnormality rate at 72 hpf of the wildtype, the expression rate of morphants was calculated according to their specific morphologic changes at 72 hpf. The most favorable Egr1-MO dose was selected to induce the highest expression rate and an acceptable lethal rate. The phenotypes were observed and photographed with a stereo dissecting microscope equipped with a digital camera. The 72-hpf morphants were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned into 5
Subjects
胚胎發育
morpholino
Egr1
斑馬魚
眼睛
eye
zebrafish
embryonic development
Type
text
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