Antioxidant activities of chicken liver hydrolysates by pepsin treatment
Journal
International Journal of Food Science and Technology
Journal Volume
49
Journal Issue
7
Start Page
1654
End Page
1662
ISSN
0950-5423
1365-2621
Date Issued
2014-07-01
Author(s)
Abstract
Summary: This study was divided into two parts: (i) an optimal hydrolysing procedure of chicken liver hydrolysates (CLHs) and (ii) the in vivo antioxidant properties of CLHs via a D-galactose-induced mouse model. A pepsin-to-raw chicken liver mass ratio (1:400, w:w) and 2-h hydrolysing period were chosen to manufacture CLHs based on yield, peptide level and antioxidant effect. Molecular masses of CLHs were lower than 10 kDa. CLH was rich in aspartic acid and glutamic acid, and also contained both manganese and selenium, which are essential cofactors of superoxide dismutase and glutathione peroxidase, respectively. The contents of cadmium, mercury, tin, and arsenic in CLHs were very low and even no detectible. Regarding the in vivo antioxidant activity of CLHs, a dosage of 1.2 g D-galactose kg-1 body weight increased (P < 0.05) 2-thiobarbituric acid reactive substances values and decreased (P < 0.05) glutathione and Trolox equivalent antioxidant capacity values, as well as superoxide dismutase, catalase, and glutathione peroxidase activities in serum and organs of mice. However, the in vivo antioxidant capacities were improved (P < 0.05) by supplementing CLHs.
Subjects
Amino acid profile
Chicken liver hydrolysate
in vitro and in vivo antioxidant effects
Mineral profile
SDGs
Publisher
Oxford University Press (OUP)
Type
journal article