Preparation of Polymethylmethacrylate microneedle array and its Property
Date Issued
2015
Date
2015
Author(s)
Lee, Hao-Yen
Abstract
Transdermal administration is a very common drug delivery route currently, but it encounters a lot of restrictions. Traditionally, transdermal administration only transfers small molecule drugs or lipophilic drugs, due to the fact that macromolecular drugs and hydrophilic drugs might be blocked by the skin’s stratum corneum. Micro-scale needles have been proposed and notified to increase skin permeability recently. This micro-scale needles called microneedles. Using this microneedle array, transdermal drug delivery shows to greatly enhance the skin permeation capacity especially for macromolecular drugs. Microneedle array is widely used in drug delivery, vaccine even DNA delivery through skin. Microneedle array has a lot of advantages as compared with conventional injection. First, microneedle’s length is about 100 ~ 200μm, which reduces the risk of the needles piercing into the dermis exposed to nerve and avoids the pains during the application on the skin. Second, microneedle administration is a self-medication and does not require the assistance from professional medical personnel. Third, microneedle array can be combined with the patch as a long-term method of administration. Using a two-stage injection, polymerization process polymeric polymethylmethacrylate (PMMA) microneedle array was formed success fully. The geometry size of PMMA microneedle array is about 1cm by 1cm. Each array of microneedle number is 6 by 6. We oberved whether the cell could be successfully attached to our material ""PMMA microneedle array."" The results show that two-stage polymerization process utilizing PMMA was feasible to produce microneedles. 3T3 cells successfully attached to the PMMA microneedle array. PMMA microneedle and metal microneedle were observed to have the similar hardness and sharpness, based on the outcomes obtained from piercing through the decellularized pigskin. In endurance tests, following ten times application of PMMA microneedle array on the decellularized pigskin, PMMA microneedle array would begin to break. After fifty times of application, PMMA microneedle array would all be broken. Under cytotoxicity tests, completely polymerized PMMA did not cause injury or toxicity to cells.
Subjects
microneedle array
cell attachment
SDGs
Type
thesis
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