Protein profilings in mouse liver regeneration after partial hepatectomy using iTRAQ technology
Journal
Journal of Proteome Research
Journal Volume
8
Journal Issue
2
Pages
1004
Date Issued
2009-02-01
Author(s)
Hsieh, Hui Chu
Chen, Yi Ting
Li, Jen Ming
Chou, Ting Yu
Huang, See Chang
Tseng, Tzu Ling
Liu, Chung Cheng
Chen, Sung Fang
Abstract
Liver is unique in its capability to regenerate after an injury- Liver regeneration after a 2/3 partial hepatectomy served as a classical model and is adopted frequently to study the mechanism of liver regeneration. In the present study, semiquantitative analysis of protein expression in mouse liver regeneration following partial hepatectomy was performed using an iTRAQ technique. Proteins from pre-PHx control livers and livers regenerating for 24, 48 and 72 h were extracted and inspected using 4-plex isotope labeling, followed by liquid chromatography fractionation, mass spectrometry and statistical differential analysis. A total of 827 proteins were identified in this study. There were 270 proteins for which quantitative information was available at all the time points in both biologically duplicate experiments. Among the 270 proteins, Car3, Mif, Adh1, Lactb2, Fabp5, Es31, Acaalb and LOC100044783 were consistently down-regulated, and Maf1a, Dnpep, Pabpc1, Apoa4, Oat, Hpx, Hp and Mt1 were up-regulated by a factor of at least 1.5 from that of the controls at one time point or more. The regulation of each differential protein was also demonstrated by monitoring its time- dependent expression changes during the regenerating process. We believe this is the first report to profile the protein changes in liver regeneration utilizing the iTRAQ proteomic technique. © 2009 American Chemical Society.
Subjects
iTRAQ | Mouse liver regeneration | Partial hepatectomy
Publisher
AMER CHEMICAL SOC
Type
journal article