Polymorphisms in DNA Repair Related Genes and Risk of Female Lung Adenocarcinoma in Northern Taiwan
Date Issued
2005
Date
2005
Author(s)
Lu, Yan-An
DOI
en-US
Abstract
Background
DNA damage is an unavoidable result by exposing to intrinsic or extrinsic factors. DNA repair is an essential mechanism for maintaining integrity and accuracy of DNA, reduced DNA repair capacity may increase the susceptibility to cancer. We examined association among ten polymorphisms of DNA repair-related genes (hOGG1 Ser326Cys, XRCC1 Arg194Thr, XRCC1 Arg280His, XRCC1 Arg399Gln, Lig1 C170A, XPD Lys751Gln, hMLH1 G-93A, XRCC3 Thr241Met, p53 Arg72Pro, and p21 Ser31Arg) and lung adenocarcinoma risk among Taiwanese female.
Methods
A total of 180 female patients with lung adenocarcinoma and 350 healthy hospital control subject recruited in northern Taiwan from July 1997 until July 2000 provided DNA samples for genotype analysis. Genetic polymorphisms were analyzed by PCR-RFLP and Taqman assays. Unconditional multivariate logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs).
Results
After adjusted for age, school year, tobacco exposure, and cooking fume exposure, the OR of developing adenocarcinoma was 2.21 (95% CI= 1.2~4.2) for XRCC1 399 Gln/Gln genotype (compared with other genotypes), 7.98 (95% CI=1.6~41.1) for XPD 751 Gln/Gln genotype (compared with other genotypes), and 1.77 (95% CI= 1.1~3.0) for p21 codon 31 Ser/Ser or Ser/Arg genotypes (compared with Arg/Arg genotype). Interactions between markers were not statistically significant, and so did interactions between markers and environmental exposures.
Conclusion
Our results suggests that the homozygous Gln alleles in codon 399 of the XRCC1 gene, homozygous Gln alleles in codon 751 of the XPD gene, and homozygous Ser alleles in codon 31 of the p21 gene might contribute to lung adenocarcinoma risk.
Subjects
女性肺腺癌
DNA修補
多形性
female lung adenocarcinoma
DNA repair
polymorphism
SDGs
Type
thesis