Antioxidative and hepatoprotective effects of Physalis peruviana extract against acetaminophen-induced liver injury in rats
Journal
Pharmaceutical Biology
Journal Volume
46
Journal Volume
46
Journal Issue
10-11
Journal Issue
10-11
Pages
724-731
Start Page
724
End Page
731
ISSN
17445116
Date Issued
2008-10
Author(s)
Abstract
The aim of this study was to examine the antioxidant activities of Physalis peruviana L. (Solanaceae) aqueous extract (PPWE) and its protective effect against acetaminophen (APAP)-induced hepatotoxicity in rats. Using different models of antioxidant assay, namely ferric thiocyanate, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and reducing power, PPWE showed a dose-dependent increase in antioxidant activities, with total antioxidant activity (IC50: 0.81 μg/ml) close to that of vitamin C (IC50: 0.89 μg/ml). APAP at 850 mg/kg significantly increased the levels of serum glutamic pyruvic transaminase (sGPT), glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (sALP). However, pre-treatment with PPWE at doses 150, 300, and 600 mg/kg body weight significantly prevented the increase in these enzymes, which are the major indicators of liver hepatitis. Biochemical assays of liver homogenate showed that PPWE at 150-600 mg/kg significantly enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) concentrations, and diminished the level of thiobarbituric acid reactive substances (TBARS). Furthermore, liver histological observation also showed an obvious amelioration in the liver cell necrosis, liver lesion, and fatty changes in PP-treated groups. High performance liquid chromatographic analysis showed that ellagic acid (ca. 0.2%) but not others could be the major component contributing to the antioxidant and hepatoprotective activities of PPWE. The present study concludes that PPWE possesses antioxidant activity and potent hepatoprotective effect against APAP-induced liver injury in rats.
Subjects
Acetaminophen
Antioxidant
Hepatoprotective
Liver injury
Physalis peruviana
SDGs
Other Subjects
1,1 diphenyl 2 picrylhydrazyl; alanine aminotransferase; alkaline phosphatase; ascorbic acid; aspartate aminotransferase; catalase; ellagic acid; glutathione peroxidase; paracetamol; Physalis peruviana extract; plant extract; superoxide dismutase; thiobarbituric acid reactive substance; unclassified drug; alanine aminotransferase blood level; alkaline phosphatase blood level; angiosperm; animal experiment; animal model; animal tissue; antioxidant activity; article; aspartate aminotransferase blood level; controlled study; drug efficacy; drug potency; fatty liver; hepatitis; high performance liquid chromatography; IC 50; liver histology; liver homogenate; liver injury; liver necrosis; liver protection; liver toxicity; male; medicinal plant; nonhuman; rat; treatment response; Physalis peruviana; Rattus; Solanaceae
Type
journal article