Comparison of hexadecane utilization by bacteria isolates from coastal water of Taiwan
Date Issued
2009
Date
2009
Author(s)
Chen, Yin-Ting
Abstract
The main composition of petroleum oil is hydrocarbon. Our laboratory isolated 29 bacterial strains which were able to utilize hexadecane as sole carbon source in 2008. After conserving strain sources for six months, six strains lost hydrocarbon-degrading ability. Four of the fastest growing bacterial strains were selected as material to study their hexadecane-utilization efficiency in three culture conditions: artificial seawater, artificial seawater with organic nutrient and filtered natural seawater. Strain HD5, HD8, HD51 and MO27 were identified as Marinobacter hydrocarbonoclasticus, Alcanivorax dieselolei, Marinobacter mobilis and Alcanivorax balearicus. Bacterial cells could adapt to new system more quickly in filtered natural seawater than in artificial seawater. Higher nitrogenous and phosphoric nutrients or organic nutrient content can improve hexadecane utilization by isolates HD5 and MO27. HD8 and MO27, classified as the same genus, had different gene sequences and number of alkB genes, and had different reactions to three culture conditions.
Subjects
hexadecane degradation
culture condition
gas chromatography
Type
thesis
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