Immunomodulatory activities of medicinal mushroom grifola frondosa extract and its bioactive constituent
Journal
American Journal of Chinese Medicine
Journal Volume
41
Journal Volume
41
Journal Issue
1
Journal Issue
1
Pages
131-144
Start Page
131
End Page
144
ISSN
0192415X
Date Issued
2013
Author(s)
Abstract
Grifola frondosa (GF), a high value medicinal mushroom in China and Japan, is popularly consumed as traditional medicines and health foods, especially for enhancing immune functions. In this study, our aim was to examine the immunomodulatory activities of GF and its bioactive compound ergosterol peroxide (EPO) in lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. At low concentrations, EPO but not other extracts showed a full protection against LPS-induced cell toxicity. EPO significantly blocked MyD88 and VCAM-1 expression, and cytokine (IL-1β, IL-6 and TNF-α) production in LPS-stimulated cells. It also effectively inhibited NF-κB activation, which was further confirmed with siRNA treatment. These results conclude that EPO may play an important role in the immunomodulatory activity of GF through inhibiting the production of pro-inflammatory mediators and activation of NF-κB signaling pathway.
Subjects
Ergosterol Peroxide
Grifola frondosa
Immunomodulatory Activity
Medicinal Mushroom
Pro-Inflammatory Mediators
THP-1 Cells
SDGs
Other Subjects
antiinflammatory agent; ergosterol peroxide; Grifola frondosa extract; immunoglobulin enhancer binding protein; immunomodulating agent; interleukin 1beta; interleukin 6; lipopolysaccharide; myeloid differentiation factor 88; peroxide; plant extract; small interfering RNA; transcription factor RelA; tumor necrosis factor alpha; unclassified drug; vascular cell adhesion molecule 1; antiinflammatory activity; article; cell protection; cell viability; controlled study; cytokine production; cytotoxicity; Grifola frondosa; human; human cell; immunomodulation; protein expression; signal transduction; Western blotting; Cells, Cultured; Cytokines; Dose-Response Relationship, Drug; Ergosterol; Grifola; Humans; Inflammation Mediators; Lipopolysaccharides; Monocytes; Myeloid Differentiation Factor 88; NF-kappa B; Plant Extracts; Signal Transduction; Vascular Cell Adhesion Molecule-1
Type
journal article