An Acyloxymethyl Ketone-Based Probe to Monitor the Activity of Glutathionylspermidine Amidase in Escherichia coli
Resource
CHEMBIOCHEM, 12(15), 2306-2309
Journal
ChemBioChem
Pages
2306-2309
Date Issued
2011
Date
2011
Author(s)
Chong, Cheong-Meng
Gao, Shijay
Chiang, Bing-Yu
Hsu, Wen-Hung
Lin, Ting-Chien
Chen, Tzu-Chieh
Lin, Chun-Hung
Abstract
Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.
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Type
journal article
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