Inhibitory Effect of proteins from Hypsizigus marmoreus on CT26 Colon Adenocarcinoma Cells and Metastasis in Balb/c Mice
Date Issued
2005
Date
2005
Author(s)
Chein, Chia-Yi
DOI
zh-TW
Abstract
Hypsizigus marmoreus (HM), one of popular dietary mushrooms, contains high protein, low calorie, and low fat and has long been consumed in Japan. Several researches indicate that HM has antitumor and antifungal activities. In the present study, HM was used to study its antitumor activity in vitro and in vivo. First, 40~80% ammonium sulfate precipitates of cold-salt water extracts from HM were prepared and incubated with mouse colon CT26 cells to observe the changes in growth inhibition (%) for 24, 48 and 72 hr by MTT assay, followed by the cell cycle arrest by a Flow Cytometry. Results showed that remarkable growth inhibition, higher than 68 %, was observed when CT26 cells were incubated with 600 μg/mL of 40~80 % ammonium sulfate precipitates of cold-salt water extracts from HM. In addition, cell cycle arrest of CT26 cells at G2/M phase and apoptosis were induced. In vivo, pulmonary metastasis of CT26 tumor on male Balb/c mice orally administrated for 13 days with doses (200, 400 and 600 mg/kg bw/day) of ammonium sulfate precipitates of cold-salt water extracts from HM and injected (iv) with 1×105 CT26 cells via a tail vein was established to observe the results. Metastasic lung nodules in experimental group fed with 200, 400 or 600 mg/kg bw/day decreased in a dose-dependent manner, revealing the significant (p<0.05) anti-metastatic effect of HM proteins. Histopathologic results in lung was consistent with the finding in vivo. Insignificant changes in renal and immunohistograph were observed in mice at those three dosages.
Subjects
鴻喜菇
小鼠大腸癌細胞
肺部轉移
Hypsizigus marmoreus
CT26 Colon Adenocarcinoma Cells
pulmonary metastasis
Type
thesis
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