Versatile and Accessible Magnetic Diagnosis Platform with Different Types of Magnetic Particles for Liquid and Solid Biopsies
Journal
International journal of molecular sciences
Journal Volume
24
Journal Issue
12
Date Issued
2023-06-20
Author(s)
Liu, Ju-Fang
Chen, Jean-Hong
Liao, Shu-Hsien
Chen, Kuen-Lin
Wei, Wen-Chun
Chen, Ting-Yuan
Chieh, Jen-Jie
Abstract
The diagnosis of liquid and solid biopsies by different instruments makes the clinic loading difficult in many aspects. Given the compositions of magnetic particles (MPs) with diverse characterizations and the innovative acoustic type of vibration sample magnetometer (VSM), the versatile, accessible magnetic diagnosis platform was proposed to meet clinical demands, such as low loading for multiple biopsies. In liquid biopsies of alpha-fetoprotein (AFP) standard solutions and subject serums, molecular concentration was analyzed from saturation magnetization by the soft type of Fe3O4 MPs with AFP bioprobe coating. In the phantom mixture simulated as bounded MPs in tissue, the bounded MPs was evaluated from the area of the hysteresis loop by hard type of cobalt MPs without bio-probes coating. Not only a calibration curve was founded for many hepatic cell carcinoma stages, but also microscale images verified the Ms increase due to magnetic protein clusters, etc. Hence, its wide populations in clinics could be expected.
Subjects
acoustic type of vibration sample magnetometer
liquid and solid biopsy
magnetic particles
soft and hard magnetic types
SDGs
Type
journal article