The Protective Effect of Nano/Submicron and Blended Lycium Barbarum on Light-induced Retinal Degeneration in a Rat Model
Date Issued
2010
Date
2010
Author(s)
Chang, Jen-Shuai
Abstract
There is no effective treatment or prophylaxis way against retinal degeneration. Light-induced retinal degeneration in a rat model was used to assess the protective effect of traditional Chinese herb “Lycium barbarum” in the retina. The Lycium barbarum can be used for clinical treatment in dogs and cats with progressive retinal degeneration, if it shows a protective role in the retina.
SD-rats were divided into four groups, namely Normal control, LI-model, Milled, and Blended. The Milled and the Blended groups were treated by feeding 250 mg/kg of Lycium barbarum once daily for 54 days including the period of light induction. The electroretinography (ERG) of all groups were assessed at the time of one day before and seven days after light induced retinal degeneration respectively. After the second ERG recording, both eyes were enucleated for histopathology and ELISA test.
The ERG results showed that there was a decline in a- and b-wave in the Milled and the Blended groups. There was a better wave amplitude preservation in the Milled group. The visual function of the test rats in the Milled group was better than that in the Blended one. The retinal histopathology results presented significant protective effect in outer retina by thickness preservation. The GSH plus GSSG concentration was significantly higher and lower MDA level compared the Milled group with the Blended one.
Briefly, the Milled and the Blended groups treated by Lycium barbarum showing protective effects in preservation of retinal thickness and function with elevated antioxidative capacity. The nano/submicron Lycium barbarum also showed better retinal protection and antioxidative effect than blended one.
Subjects
AMD
light damage
antioxidation
nano/submicron
Lycium barbarum
retina
Type
thesis
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