Investigation on the Active Hypoglycemic Components of Siraitia Grosvenori by Glucose Uptake Assay in 3T3-L1 Adipocytes
Date Issued
2012
Date
2012
Author(s)
Chang, Yao-Yun
Abstract
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Muscle and fat cells are both insulin-stimulated target tissues which regulate the blood glucose concentration. Glucose uptake in target tissues is a critical step in maintaining glucose homeostasis and clearing the postprandial glucose load. Therefore, evaluation of glucose uptake ability in cells plays a fundamental role in diabetes research. Siraitia grosvenori (SG), also called Luo-Han-Guo (LHG), is a fruit used in traditional Chinese medicine and also used as food additives. Besides its sweet taste, studies showed that LHG-extract exhibited an antidiabetic effect on both in vivo and in vitro trials. The aim of this study is to find the active compounds with hypoglycemic potential in LHG by glucose uptake assay in 3T3-L1 cells. LHG crude extract increased glucose uptake in a dose-dependent manner, and after solvent partition of crude extract, the ethyl acetate fraction showed the best glucose uptake activity. There were three components (S1, S2 and S3) separated from EA fraction by column chromatography, and all of them can significantly increase glucose uptake activity in 3T3-L1 adipocytes. Among them, S1 showed the best activity in basal glucose uptake. In combination with insulin, however, S1 slightly reduced insulin-stimulated glucose uptake. By western blot analysis, S1 enhanced the phosphorylation level of Akt. S2 was found to have a promotion effect on both basal and insulin-stimulated glucose uptake. In conclusion, S1 had an insulin-mimetic action and S2 showed synergistic effect with insulin in 3T3-L1 adipocytes.
Subjects
Siraitia grosvenori
anti-diabetic
glucose uptake
3T3-L1 adipocytes
SDGs
Type
thesis
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