The Protective Role of Heme Oxgenase in Ischemic Preconditioning
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
賴逸儒
DOI
912314B002348
Abstract
Background: Hypoxic preconditioning (HP) confers cytoprotection against
ischemia/reperfusion （I/R）injury, this effect is in part due to the induction of
heme oxygenase-1. This experiment evaluates liver cell damage after I/R
injury in HP rats.
Methods： HP rats were prepared by exposure (15hours day-1) to an altitude
chamber (5500m) for 2 weeks. Partial hepatic ischemia was produced in the
left lobes for 45 minutes followed by 180 minutes of reperfusion.
Zinc-protoporphyrin IX(ZnPP), a specific inhibitor of HO enzymatic activity, was
subcutaneously injected 1 hour before the I/R injury in separate groups of
sea-level （SL）control and HP rat. Serum alanine transaminase (ALT) levels,
liver HO-1 mRNA and protein, and HO enzymatic activity were measured.
Results：Heme oxygenase-1 (HO-1) was induced in the livers of rats exposed
to HP. The levels of HO-1 mRNA and protein were obviously overexpressed
after two weeks of hypoxic preconditioning. HP diminished the elevation of
serum ALT levels after I/R injury （83.7±4.9 U L-1）when compared with SL
controls （280.8±19.4 U L-1） and HP+ ZnPP pre-treated groups（151.3±4.4 U
L-1）. The heme oxygenase activity in treated rats also correlated these results
（237.9±19.8 pmol mg-1 protein hr-1 for the HP group, 164.3±12.7 pmol mg-1
protein hr-1 for the HP+ ZnPP, and 182.6±8.9 pmol mg-1 protein hr-1 for the SL
controls.
Conclusions：Our results indicated that the induction of HO-1 in hypoxic
preconditioning played a protective role against hepatic I/R injury.
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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