Isoelectric focusing-reversed phase liquid chromatography polymer microchip with integrated high-pressure valves
Journal
ASME International Mechanical Engineering Congress and Exposition, Proceedings
Journal Volume
12
Journal Issue
PART B
Pages
961-963
Date Issued
2010
Author(s)
Abstract
A cyclicolefin polymer (COP) microchip supporting parallel 2-D peptide separations is described. By combining isoelectric focusing (IEF) as a first dimension and parallel reversed-phase liquid chromatography (RPLC) as a second dimension, the system enables efficient high-throughput fractionation prior to mass spectrometry in support of peptide mass fingerprinting for global proteomic analysis from highly limited specimens. The IEF-RPLC chip incorporates high-pressure micro shut-off valves, allowing uniform sample transfer and gradient elution from each micro LC column, and ensuring hydrodynamic isolation between the separation dimensions. The utility of the initial microchip is demonstrated by separation of a fluorescein labeled bovine serum albumin tryptic digest in a chip containing a five channel RPLC array.
SDGs
Type
conference paper