Genetic and functional analyses describe a novel 730delG mutation in the KEL gene causing K-0 phenotype in a Taiwanese blood donor
Resource
TRANSFUSION MEDICINE, 21(5), 318-324
Journal
Transfusion Medicine
Pages
318-324
Date Issued
2011
Date
2011
Author(s)
Abstract
Aims/Objectives: The purpose of this study was to explore the molecular basis of the K 0 phenotype of a Taiwanese blood donor found to have anti-Ku alloantibodies. Background: With respect to Kell blood group antigens, almost all Taiwanese have the (K-, k+) phenotype. Materials and Methods: Alloantibody identification and KEL antigen typing were performed. Enzymatic function assays were carried out to detect the Kell glycoprotein on RBCs. The KEL genes were sequenced to detect genetic variation. To determine the origin of this novel allele, family studies were conducted. Results: The alloantibody was identified as anti-Ku. The donor was typed K 0. The KEL gene-sequencing data revealed that this K 0 donor is a compound heterozygote with two different null alleles. He bears a novel 730delG mutation in one allele. Family studies suggested that the donor inherited the 730delG mutation from his father. The endothelin-converting activity assay indicated that his RBCs had no functional Kell glycoprotein. Other family members who had only one null allele with the 730delG mutation had the phenotype (K-, k+). Conclusion: For blood transfusion safety, it is important to establish an effective screening algorithm to identify rare phenotypes, such as the K 0 phenotype, and to establish a database of rare blood groups. ? 2011 The Authors. Transfusion Medicine ? 2011 British Blood Transfusion Society.
SDGs
Other Subjects
alloantibody; blood group antigen; blood group kell antigen; glycoprotein; Ku antigen; unclassified drug; allele; article; blood donor; blood group k0; blood group Kell system; case report; gene; gene sequence; genetic analysis; genetic variability; heterozygote; human; kel gene; phenotype; sequence analysis; Taiwan; Blood Donors; Blood Transfusion; DNA Mutational Analysis; Family; Heterozygote; Humans; Isoantibodies; Kell Blood-Group System; Mutation; Phenotype; Taiwan
Type
journal article
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