Effect of Oxygen on the Butanol Production of Clostridium Thermosaccharolyticum
Date Issued
2009
Date
2009
Author(s)
Chao, Hsuan-Shen
Abstract
In this research, we provided an alternative method to enhance the production of butanol, and scrutinized and examined the reasons why purging with trace amount of air to sporulation culture of Clostridium thermosaccharolyticum synchronized by a sequential dilution process can increase the amount of butanol.xperimental results indicated that the amount of solvent production (ethanol and butanol) increased by air injection and increasing the frequency of air injection in Hungate tube at 36℃. With 0.1 mL-air/4hr condition, the production of solvents was found to be 14.0 g/L（butanol 11.1 g/L） after 8 days cultivaion. At 56℃, with 0.3 mL-air/4hr condition, the production of solvents was found to be 9.2 g/L（butanol 7.5 g/L） after 3 days cultivaion.e used the concept of oxygen molar ratio to scale-up the sporulation culture into 3L fermentor. In 3L fermentor at 36℃, the production of solvents was found to be 15.8 g/L（butanol 11.3 g/L） after 8 days cultivaion and purging with 6.9 mL-air/hr condition, and the molar ratio of oxygen was 0.0800. In 3L fermentor at 56℃, the production of solvents was found to be 14.8 g/L（butanol 7.9 g/L） after 3 days cultivaion with 6.9 mL-air/hr condition.ecause of the substrate inhibition on soluble carbon source, the production of butanol did not increase when increasing the concentration of calcium gluconate. However, increasing the amount of insoluble carbon source such as, paraffin oil would increase the production of solvents. At 56℃, the production of solvents was found to be 15.5 g/L（butanol 9.9 g/L） with 0.2 mL-air/8hr condition. At 36℃, the production of solvents was found to be 16.7 g/L（butanol 10.2 g/L） with 0.2 mL-air/hr condition. We found the production of butanol decreased while a series of sporulation culture prepared at descending the initial pH value from 6.5 to 4.0. Besides, increasing the initial ratio of elongated cell or the cell concentration, the production of solvents did not increase with air injection at 36℃or 56℃.
Subjects
Clostridium thermosaccharolyticum
butanol
oxygen stress
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-D91524002-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):a0351d34e28d1016c3f9c0d14a15a2f6