Guiding stem cell differentiation and proliferation activities based on nanometer-thick functionalized poly-p-xylylene coatings
Journal
Coatings
Journal Volume
11
Journal Issue
5
Date Issued
2021-05-01
Author(s)
Wu, Chih Yu
Christy, Jane
Chang, Nai Yun
Wenny, Chiu Y.-C.
Chiu, Yu Chih
Yang, Yen Ching
Chen, Po Chun
Wang, Peng Yuan
Abstract
Modifications of biomaterials based on the combination of physical, chemical, and bio-logical cues for manipulating stem cell growth are needed for modern regenerative medicine. The exploitation of these sophisticated modifications remains a challenge, including substrate limitation, biocompatibility, and versatile and general cues for stem cell activities. In this report, a vapor-phase coating technique based on the functionalization of poly-p-xylylene (PPX) was used to generate a surface modification for use with stem cells in culture. The coating provided the ability for covalent conjugation that immobilized bone morphogenetic protein 2 (BMP-2) and fibroblast growth factor 2 (FGF-2), and the modified coating surfaces enabled direct stem cell differentiation and controlled proliferation because of the specific activities. The ligations were realized between the growth factors and the maleimide-modified surface, and the conjugation reactions proceeded with high specificity and rapid kinetics under mild conditions. The conjugation densities were approximately 140 ng·cm−2 for BMP-2 and 155 ng·cm−2 for FGF-2. Guiding the activities of the human adipose-derived stem cells (hADSCs) was achieved by modifying surfaces to promote the hADSC differentiation capacity and proliferation rate. The reported coating system demonstrated biocompatibility, substrate-independent conformity, and stability, and it could provide an effective and versatile interface platform for further use in biomedical applications.
Subjects
Biointerface | CVD polymerization | Growth factor | Stem cells | Surface modification
Biointerface; CVD polymerization; Growth factor; Stem cells; Surface modification
SDGs
Type
journal article