Isolation, Identification and Sequence Analysis of Avian Reticuloendotheliosis Viruses from Goose in Taiwan
Date Issued
2008
Date
2008
Author(s)
Lin, Chia-Yao
Abstract
Many reticuloendotheliosis virus (REV) associated studies have been focused on chicken or turkey, but rarely on goose. The purpose of this study was to investigate theEV occurrence in geese in Taiwan. PCR and serum neutralization test were used to detect REV infection. Nine REV isolates from a white Roman goose farm were isolated by inoculating buffy coat onto the DF-1 cell line. The complete proviral sequence of one REV was determined. The relationship of gene sequences of four Taiwanese REVs were compared with the reference REVs by the phylogenetic analysis. The percent of nucleotide identity of gag and pol ranged from 96.9 to 99.9% and 96.7 to 99.9%, respectively. The percent of nucleotide identity of env ranged from 95.9 to 100%. The LTR was the most divergent region of genome with 74.9 to 99.8% nucleotide identity containing deletion and insertion. It indicates that this isolate is closely related to other REVs and the gene diversity among REV isolates in different avian hosts is minimal. For developing a blocking enzyme-linked immunosorbent assay (ELISA) to detect antibody in waterfowls, the concentrated and purified REV was used as coating antigen and monoclonal antibody was labeled with horseradish peroxidase as tracer. The optical density value was close to 1 on the condition that 600 ng of REV per well was coated with 50-fold diluted tracer. The REV detection by PCR showed 28.8% positive rate and antibody detection by serum neutralization test showed 60.3% positive rate in four goose farms. Thus REV infection is common in geese in Taiwan.
Subjects
avian reticuloendotheliosis virus
phylogenetic analysis
neutralization test
monoclonal antibody
virus isolation
goose
avian oncogenic disease
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95629019-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):97180ce78c60e87bcab5139c51671c49