The product of the ABC half-transporter gene ABCG2 (BCRP/MXR/ABCP) is expressed in the plasma membrane
Journal
Biochemical and Biophysical Research Communications
Journal Volume
271
Journal Issue
1
Pages
42-46
Date Issued
2000
Author(s)
Abstract
The products of the ABC gene family can be generally classified as either full-transporters of half-transporters. Full-transporters are expressed in the plasma membrane, whereas half-transporters are usually found in intracellular membranes. Recently, an ABC half-transporter, the ABCG2 gene product Breast Cancer/Mitoxantrone Resistance Protein (BCRP/MXR), has been shown to cause mitoxantrone and topotecan resistance. The purpose of this study was to determine the expression and the intracellular localization of this protein in various drug-resistant cell lines. BCRP/MXR expression was determined by Western blot and immunohistochemistry. This protein is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane, instead of to intracellular membranes as seen with all other known half-transporters. Therefore, BCRP/MXR is unique among the ABC half-transporters by being localized to the plasma membrane. (C) 2000 Academic Press.
SDGs
Other Subjects
ABC transporter; mitoxantrone; topotecan; article; breast cancer; cell membrane; drug resistance; gene overexpression; human; human cell; immunoblotting; immunohistochemistry; intracellular membrane; priority journal; protein expression; protein localization
Publisher
Academic Press Inc.
Type
journal article