YC-1對於間隔細胞cyclin,cyclin dependent kinase inhibitor表現、分佈及cyclin dependent kinase活性的調控
Date Issued
2004
Date
2004
Author(s)
姜文智
DOI
922314B002363
Abstract
This study was designed to investigate the effect of YC-1 upon the proliferation of
rat mesangial cells and its underlying mechanism. YC-1 inhibited cell proliferation
and DNA synthesis in a dose and time-dependent manner. Flow-cytometry cell-cycle
studies revealed that YC-1 prevented the entry of cells from G1 into S phase. The
expression of cyclin D1 and the kinase activity of cyclin D1/CDK 4 were lower
within YC-1-treated cells, revealed by Western blotting, Northern blotting and kinase
assays. YC-1 did not increase the intracellular cGMP concentration in mesangial cells.
Inhibitors of sGC, PKG, or PKA also did not reverse the inhibitory effect elicited by
YC-1, while co-treatment with p38 MAPK inhibitor could partially reversed the
suppressive effect. Conclusion: YC-1 inhibited proliferation and induced cell-cycle
arrest by the reduction of cyclin D1 synthesis and cyclin D1/CDK4 kinase activity.
This effect acts partially through p38 MAPK signal transduction activation and is
independent of cGMP-signaling pathways.
Subjects
YC-1
mesangial cells
cyclin D1
proliferation
p38 mitogen-activated
protein kinase
protein kinase
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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