Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2
Resource
Journal of Biomedical Science 15 (1): 15-27
Journal
Journal of Biomedical Science
Journal Volume
15
Journal Issue
1
Pages
15-27
Date Issued
2008
Author(s)
Lai, Chih-Yun
Hu, Hsien-Ping
King, Chwan-Chuen
Wang, Wei-Kung
Abstract
While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causal agent of the most important arboviral diseases in this century. In this study, we successfully established a cell line stably expressing the precursor membrane/envelope (PrM/E) proteins of dengue virus type 2 (DENV2), which can package a DENV2 replicon with deletion of PrM/E genes and produce single-round infectious VLPs. Moreover, it can package a similar replicon of different serotype, dengue virus type 4, and produce infectious chimeric VLPs. To our knowledge, this study reports for the first time replicon-containing VLPs of dengue virus. Moreover, this convenient system has potential as a valuable tool to study encapsidation of dengue virus and to develop novel chimeric VLPs containing dengue virus replicon as vaccine in the future. ? 2007 National Science Council.
Subjects
Dengue virus (DENV); Replicon; Virus-like particles (VLPs)
SDGs
Other Subjects
dengue vaccine; E protein; envelope protein; glycylalanylglycylcysteinylalanylglycylalanylthreonylcysteinyl threonylcysteinylthreonylglycylglycylalanylalanylalanylalanylalanyl threonylglycyl; protein C; threonylthreonylthreonylcysteinylcysteinylglycylglycylglycyl cysteinylglycylcysteinylglycylcysteinylcysteinylglycylalanylthreonyl threonylthreonylalanylglycylglycyl; unclassified drug; virus protein; article; cell culture; controlled study; dengue; Dengue virus; gene deletion; gene expression; nonhuman; priority journal; replicon; serotype; virus like agent; virus replication; Base Sequence; Cell Line; Chimera; Dengue Virus; DNA Primers; DNA, Viral; Gene Expression; Genes, Viral; Humans; Replicon; Viral Envelope Proteins; Viral Matrix Proteins; Virion; Virus Replication; Dengue virus; Dengue virus type 2; Dengue virus type 4
Type
journal article
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