Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells
Resource
Oncol. Rep., 30(3), 1497-1505
Journal
Oncology Reports
Pages
1497-1505
Date Issued
2013
Date
2013
Author(s)
Yan K.-H.
Lee L.-M.
Hsieh M.-C.
Yan M.-D.
Yao C.-J.
Chang P.-Y.
Chen T.-L.
Chang H.-Y.
Lai G.-M.
Chuang S.-E.
Abstract
Methotrexate (MTX) has been widely used for the treatment of cancer and rheumatoid arthritis (RA). Aspirin (ASA) is a non-selective cyclooxygenase (COX) inhibitor that contributes to the treatment of inflammatory conditions such as RA. It has been observed that the antitumor effect of ASA can be attributed to inhibition of cell cycle progression, induction of apoptosis and inhibition of angiogenesis. In the present study, we revealed that the treatment with a combination of MTX and ASA resulted in antagonism of the cytotoxic effect as demonstrated by SRB and colony formation assays. ASA alleviated the MTX-mediated S phase accumulation and recovered the G1 phase. MTX-mediated accumulation of the S phase marker cyclin A was also alleviated by ASA. Notably, FAS protein levels were upregulated by MTX in A549 cells. The antagonism of MTX efficacy caused by ASA was accompanied by altered expression of caspase-3, Bcl-2 and FAS but not dihydrofolate reductase (DHFR). This suggests that the alteration of caspase-3, Bcl-2 and FAS was involved in the antagonism between ASA and MTX. Exogenously added folic acid reversed the MTX-mediated DHFR inhibition following either MTX or MTX + ASA treatments. Most importantly, we demonstrated for the first time that the commonly used non-steroidal anti-inflammatory drug for headache ASA and possibly other COX-1/2 inhibitors can produce a strong antagonistic effect on the growth inhibition of lung cancer cells when administered in combination with MTX. The clinical implication of our finding is obvious, i.e., the clinical efficacy of MTX therapy can be compromised by ASA and their concomitant use should be avoided.
Subjects
aspirin
methotrexate
antagonism
SDGs
Other Subjects
acetylsalicylic acid; caspase 3; cyclin A; dihydrofolate reductase; Fas antigen; methotrexate; protein bcl 2; antineoplastic activity; article; bioaccumulation; cancer cell; cancer cell culture; cell cycle G1 phase; cell cycle S phase; cell proliferation; cell survival; colony formation; controlled study; drug antagonism; drug cytotoxicity; drug efficacy; enzyme inhibition; human; human cell; in vitro study; lung cancer; priority journal; protein expression; upregulation; Adenocarcinoma; Anti-Inflammatory Agents, Non-Steroidal; Antimetabolites, Antineoplastic; Apoptosis; Aspirin; Blotting, Western; Cell Cycle; Cell Proliferation; Drug Antagonism; Humans; Lung Neoplasms; Methotrexate; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tumor Cells, Cultured
File(s)![Thumbnail Image]()
Loading...
Name
index.html
Size
23.16 KB
Format
HTML
Checksum
(MD5):3900e1d80ee6b3feff0e859f99f063b8