Understanding regulatory network among transcription factors on expression of CrPR1a and CrLOX2 in Catharanthus roseus
Date Issued
2014
Date
2014
Author(s)
Lo, Hsiao-Fu
Abstract
Phytoplasmas are wall-less prokaryotic obligate plant pathogens that are restricted in phloem. They have caused serious economic loss worldwide. In periwinkle, a PR gene-related defense mechanism was found to be related with defense against phytoplasmas. In addition, CrLOX2, which is a jasmonate-responsive and biosynthesis gene, is also induced by the phytoplasma infection. Because regulators of CrPR1a and CrLOX2 may participate in defense against phytoplasmas, both genes were used as marker genes to construct a defense regulatory network using VIGS. We identified 55 groups of 723 transcription factors from periwinkle transcriptome database generated by NTU research team. Among them, we selected those potentially related to biotic or abiotic stresses, including a total of 6 groups, 152 transcription factors, which are ARF (18)、Aux-IAA (8)、bZIP (27)、C2H2 (40)、MYB (45)、MYB-related (14). Results showed that three ARF, one Aux-IAA, and one bZIP transcription factors were identified to regulate the expression of CrPR1a. Their expressions under phytoplasma infection, different hormone treatments, and CrNPR1 or CrNPR3 gene silencing were examined. Results showed that IAA-6, which is a positive regulator of CrPR1a, was induced under phytoplasma infection. IAA-6 is also positively regulated by a negative regulator of CrPR1a, bZIP-7. In addition, ARF-12, which is a positive regulator of CrPR1a, is positively regulated by another positive regulator of CrPR1a, ARF-13. Through the research, we have discovered novel transcription factors regulating CrPR1a and constructed a transcriptional regulatory network. This might help us to better understand the interaction between phytoplasma and its host plant , which would facilitate the development of more effective control strategies.
Subjects
CrPR1a
CrLOX2
植物菌質體
病毒誘導基因靜默
轉錄因子
網絡建構
Type
thesis
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