木質醋酸菌轉型系統之建立與應用﹝3/3﹞
Date Issued
2002
Date
2002
Author(s)
DOI
902313B002265
Abstract
Acetobacter xylinum is widely been used in food industry as it can ferment
vegetables and fruits to produce the second metabolite— bacterial cellulose which is
called nata in the market. However, nata is not easy for coloring, so it is essential to
increase its additional value for foodstuff use. In the first part of this study,
Acetobacter xylinum was transformed with pHY300PLK and pUCD2 for the finding
of optimum transformation efficiency. The result showed that electroporation has the
best transformation efficiency, with the conditions of 25 mF capacitance, 329 Ù ohms
resistance, pulse lengths of 8.2 ms, and 2.5 kV. pHY300PLK and pUCD2 were found
to have efficiency of 1.63 x 104 and 1.27 x 104 transformants / mg DNA, respectively.
Cellulose binding protein gene (cbp A) and carotenoids synthesis gene were later
cloned into plasmid pHY300PLK for further expression in Escherichia coli BL21 and
Acetobacter xylinum CCRC14101 and protein activity been detected by Western
blotting. Another plasmid pWSW8, is constructed from pUC18, an innate plasmid in
Acetobacter xylinum and carotenoids synthase gene was later constructed for the
increasing of stability and capability of replication, and promoting expression of
foreign gene by the lac promoter within pWSW8.
Subjects
Acetobacter xylinum
transformation cloning
Publisher
臺北市：國立臺灣大學園藝學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
902313B002265.pdf
Size
1.54 MB
Format
Adobe PDF
Checksum
(MD5):3e7beef2a1798d3cf9733545e429111e