Evaluation of Chondrogenic Differentiation Potential of Human Mesenchymal Stem Cells Grown in Tissue Engineering Scaffolds
Date Issued
2012
Date
2012
Author(s)
Lin, Ting-Yu
Abstract
The chondrogenesis differentiation potential of mesenchymal stem cells (MSC) isolated from four different human tissues were compared on two biomaterials, type II collagen-hyaluronan composite (CII-HA) films and small intestinal submucosa (SIS) sheets. The four human MSCs were bone marrow-derived mesenchymal stem cells (BMSC), adipose-derived adult stem cells (ADAS), gingival fibroblasts (GF) and placenta-derived mesenchymal stem cells (PDMC). The CII-HA composite films or three-dimensional (3D) scaffold were fabricated in this study. Upon TGF-β3 induction, PDMC demonstrated the best chondrogenesis differentiation potential on both materials, followed by GF. PDMC and GF were further seeded in CII-HA composite scaffolds and 8-layer SIS scaffolds for evaluation of neocartilage formation in vitro. After 28 days, CII-HA composite scaffolds seeded with either MSCs were surfaced with a cartilaginous-like layer. Histology also showed better neocartilage formation when MSCs were grown in CII-HA composite scaffolds. NOD SCID mice subcutaneous implantation further confirmed that the combination of PDMC and CII-HA composite scaffolds promoted the formation of tissue-engineered cartilage.
Subjects
Type II collagen
Hyaluronan
Small intestinal submucosa
Placenta-derived mesenchymal stem cells
Gingival fibroblasts
NOD SCID mice
Type
thesis
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