The Development of Confocal Fluorescence Microscopy and Its Application on the Measurement of CE chip

In recent years, CE has been applied in the field of gene engineering and the research of protein and biochemistry. In July, 2000, American Sanitation Bureau announced that they had made the first stage of Human Genome Project. They ascribed the success to the development and progress of CE because the technique had the advantages of saving amount and time. In the past, the instruments of conducting CE were usually big and thus they are not easy to be used in other fields. Recently, the CE chip that integrates MEMS and analysis science is gradually concerned because it has the advantages of requiring fewer samples, having a small volume, a lower price, and a higher efficiency. And the main purpose of this research aims at setting up a Coufocal Fluerescence Microscopy System and then using this system to assess some other particular systems. In the resolution test, under the circumstances that the wavelength is 532 nm and the aperture is 0.65, the trausverse and axial resolution of this system is about 0.99um and 2.43um. We will apply this Coufocal Fluorescence Microscopy System to the DNA test of CE chip and discuss the influence of the electroosmotic and electrophoresis in electronic dynamics and the effect of polymer molecule screen upon the DNA separation.