Analytical methods for cholesterol quantification
Journal
Journal of Food and Drug Analysis
Journal Volume
27
Journal Issue
2
Pages
375-386
Date Issued
2019
Author(s)
URI
Abstract
Cholesterol is an important lipid molecule in cell membranes and lipoproteins. Cholesterol is also a precursors of steroid hormones, bile acids, and vitamin D. Abnormal levels of cholesterol or its precursors have been observed in various human diseases, such as heart diseases, stroke, type II diabetes, brain diseases and many others. Therefore, accurate quantification of cholesterol is important for individuals who are at increased risk for these diseases. Multiple analytical methods have been developed for analysis of cholesterol, including classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS). Strategy known as ambient ionization mass spectrometry (AIMS), operating at atmospheric pressure, with only minimal sample pretreatments for real time, in situ, and rapid interrogation of the sample has also been employed for quantification of cholesterol. In this review, we summarize the most prevalent methods for cholesterol quantification in biological samples and foods. Nevertheless, we highlight several new technologies, such as AIMS, used as alternative methods to measure cholesterol that are potentially next-generation platforms. Representative examples of molecular imaging of cholesterol in tissue sections are also included in this review article. ? 2018
Subjects
AIMS; Cholesterol; DART; DESI; Mass spectrometry; Quantification
SDGs
Other Subjects
cholesterol; ambient ionization mass spectrometry; analytic method; cholesterol blood level; desorption electrospray ionization mass spectrometry; electrospray mass spectrometry; enzymatic assay; gas liquid chromatography; human; lipid analysis; mass spectrometry; matrix-assisted laser desorption-ionization mass spectrometry; nonhuman; nutritional science; quantitative analysis; real time mass spectrometry; Review; tandem mass spectrometry; tissue section
Type
journal article