Simultaneous visualization of the subfemtomolar expression of microRNA and microRNA target gene using HILO microscopy
Journal
Chemical Science
Journal Volume
8
Journal Issue
9
Pages
6670-6678
Date Issued
2017
Author(s)
Abstract
expression levels was observed in miR-10b* precursor-transfected cells and miR-10b* inhibitor-transfected cells. The trends of the miRNA alterations obtained using HILO microscopy completely matched the RT-qPCR data and showed remarkable reproducibility (the coefficient of variation [CV] = 0.86%) and sensitivity (<1.0 fM). This proposed imaging method appears to be useful for the simultaneous visualisation of ultra-trace amounts of miRNA and target mRNA and excludes the procedures for RNA extraction and amplification. Therefore, the visualisation of miRNA and the target mRNA should facilitate the exploration of the functions of ultra-trace amounts of miRNA in fixed cells in biological studies and may serve as a powerful tool for diagnoses based on circulating cancer cells.
SDGs
Publisher
Royal Society of Chemistry
Type
journal article