Immunohistochemical diagnosis of mouse hepatitis virus and Mycoplasma pulmonis infection with murine antiserum
Journal
Journal of Comparative Pathology
Journal Volume
131
Journal Issue
2-3
Pages
214-220
Date Issued
2004
Author(s)
Abstract
This study established a modified alkaline phosphatase-labelled avidin-biotin-complex (ABC-AP) method for diagnosis of mouse hepatitis virus (MHV) and Mycoplasma pulmonis infection from formalin-fixed, paraffin wax-embedded sections, murine antibody-positive serum being used as the primary reagent. With this method, MHV antigen in cAnNCrj.Cg- Foxn1nu/Foxn1nu mice and M. pulmonis antigen in Wistar rats were immunolabelled in tissue sections. MHV antigen was clearly detected in samples of liver, stomach, caecal and colonic mucosa, and spleen. M. pulmonis antigen was demonstrated on the luminal surface of bronchiolar epithelial cells. This method may prove useful in diagnosis when commercial antisera are unavailable or when immunosuppression prevents serological diagnosis. ? 2004 Elsevier Ltd. All rights reserved.
Subjects
Bacterial infection; Mouse; Mouse hepatitis virus; Mycoplasma pulmonis; Viral infection
SDGs
Other Subjects
alkaline phosphatase; antiserum; avidin; bacterial antigen; biotin; formaldehyde; paraffin; reagent; virus antigen; animal cell; animal experiment; animal tissue; antibody blood level; antibody labeling; antigen detection; article; bacterial infection; bronchus mucosa; cecum; cell surface; colon mucosa; complex formation; controlled study; diagnostic procedure; epithelium cell; female; immune deficiency; immunohistochemistry; liver; male; mouse; mouse strain; Murine hepatitis coronavirus; Mycoplasma pulmonis; nonhuman; rat; rat strain; serology; spleen; stomach; tissue fixation; tissue section; virus hepatitis; Animals; Antigens, Bacterial; Antigens, Viral; Coronavirus Infections; Female; Immune Sera; Immunohistochemistry; Male; Mice; Murine hepatitis virus; Mycoplasma Infections; Mycoplasma pulmonis; Rats; Bacteria (microorganisms); Murinae; Murine hepatitis virus; Mycoplasma; Mycoplasma pulmonis; Rattus norvegicus
Type
journal article