Shear-wave elasticity measurement of three-dimensional cell-matrix
Date Issued
2015
Date
2015
Author(s)
Charng, Ching-Che
Abstract
Microenvironment stiffness was formed by cell and ECM. In the cell development and the tumor progression, cell would sense the local stiffness and then adapt their behavior such as cell differentiation or cancer cell remodeling substrate stiffness even metastasis. Stiffness has been proved its importance in 2D condition. However, we lack suitable approach and platform to dynamically measure the stiffness in 3D condition. Supersonic-based shear wave elasticity imaging has great spatial resolution, temporal resolution, and measure in non-invasive way. In our present work, we developed a device fabricated by PDM to bind 3D collagen gel and matrigel to do 3D cell culture and measure cell activity. We generate the ultrasound radiation force by 20 MHz push transducer and monitor the shear wave propagation by 40 MHz image transducer. At first, we use rheometer as a gold standard technique to validate our system. Secondly, we test our measuring parameter, and found that gel thickness, binding-base, temperature would not significantly affect our measurement. In addition, we could discern spatial patterned heterogeneous agarose gel. Moreover, we found that H9C2 myoblast would stiffen the gel in a half day and it would rebound by BDM blocking which proved that the stiffness change was correlated with cell contraction. On the other hand, CL1-5 would have different behavior in two different concentration of collagen gel. In 1 mg/mL gel, collagen gel would be stiffer but not in 2 mg/mL gel that it may involve ECM remodeling.
Subjects
3D cell culture
shear wave elasticity imaging
ECM remodeling
collagen
stiffness
SDGs
Type
thesis
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