Study of Integrating Optical Coherence Tomography and Confocal Fluorescence Microscopy
Date Issued
2010
Date
2010
Author(s)
Cheng, Nai-Chia
Abstract
Optical coherence tomography (OCT) and confocal fluorescence miroscopy (CFM) are the two most important noninvasive optical diagnosis techniques nowadays. OCT provides structural information in tissue by detecting variation of scattering property of living sample, where CFM measures the intensity of fluorescence signals point-to-point in three-dimensional (3D) space, and achieves high resolution functional imaging. The two techniques provide not only spatial resolution with micrometer scale but also complementary image information. Therefore, it is an very important research object to integrate the two techniques into a two modal optical imaging system up to now.
In this thesis,we use a 445 nm laser diode as excitation light of CFM and pumping source of broadband amplified spontaneous emission (ASE) generated by Ce3+:YAG double-cladding crystal fiber, successfully construct a two modal imaging system with 3D resolving power. By using Ce3+:YAG particle as sample, we verify the ability of two modal image integration, and this preliminary result demotrate the application potential of our system on medical and life science.
We also perform 3D imaging on cultured human pulmonary adenocarcinoma cells and mesenchymal stem cells. In a homemade cellular structure, we find that cancer cells have the tendency of approaching stem cells when they get closer. By using fluorescence protein transfected cells as sample, we can make a much more quantitative analysis of this phenomenon with the help of CFM in our system in the future.
Subjects
Optical coherence tomography
Confocal fluorescence microscopy
Crystal fiber
SDGs
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