Mass production of injectable dermal papilla spheroids of controllable size on poly(vinyl alcohol) surface for hair follicle regeneration
Date Issued
2010
Date
2010
Author(s)
Lin, Wei-Ting
Abstract
Hair loss is a common disorder that often causes more intense influence in psychological than physiological aspect. Expensive hair transplantation can only redistribute remaining hair to bald area and can no be applied to patients with extensive hair loss. Regarding this, hair follicle (HF) dermal papilla(DP) cells beome a promising solution due to their ability to induce HF regeneration.
DP cells exhibit the potential to guide the transdifferentiation of epidermal cells into follicular structure only when they are kept in multicellular aggregates. A number of tissue engineering approaches have been developed to produce three-dimensional multicellular spheroids. Methods depending on intercellular self-assembly of cells attached to biomaterials encounter difficulty in harmlessly harvesting multicellular spheroids with ease. On the other hand, suspension culture methods can result in variation in spheroid sizes.
According to the poor adhesiveness of cells to hydrophilic surface, we culture DP cells in 96-well PCR plate coated with poly(vinyl alcohol)(PVA). Due to high collision frequency in limited space, we can obtain size-controllable human or rat DP spheres rapidly within 24 hours in culture by varying the numbers of cells seeded.
Further analysis reveals that more than 80% of cells within the spheres are viable and this viability is higher than that in spheres obtained by hanging drop method. DP spheres have a compact structure in histology and preserve DP signature gene expression profile that is related to the HF induction ability. We also demonstrate that the structure and cellular viability of DP spheres remain intact after they are injected through a cell delivery injection apparatus. Functionally, both human and rat DP spheres can successfully induce the HF regeneration in HF regeneration assay. The HF induction ability is varied when DP spheres of various sizes are tested and our results suggest that a minimum cell number is required within each DP sphere to obtain a higher HF induction efficiency.
We successfully set up an easy method for fast generation of size-controllable DP cell spheres and this method is of scalable potential with automatic equipment. Our system of mass preparation of trichogenic DP spheres can also be applied to clinical investigation for HF regeneration in the future.
Subjects
hair follicle regeneration
three dimension culture
dermal papilla
poly(vinyl alcohol)
size control
Type
thesis
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