Outcomes of assisted reproductive techniques for HIV-1-discordant couples using thawed washed sperm in Taiwan: Comparison with control and testicular sperm extraction/microscopic epididymal sperm aspiration groups
Journal
Journal of the Formosan Medical Association
Journal Volume
110
Journal Issue
8
Pages
495-500
Date Issued
2011
Author(s)
Abstract
Background/Purpose: An increasing number of human immunodeficiency virus-1 (HIV-1)-discordant couples in Taiwan have been seeking fertility help. We conducted the first clinical trial in Taiwan of assisted reproductive technology (ART) using sperm washing and viral load measurement. Methods: From 2005 to 2009, we performed 22 ART cycles on 14 HIV-1-discordant couples. The sperm washing involved density gradient centrifugation followed by swim-up method. HIV-1 RNA was checked by real-time reverse transcription-polymerase chain reaction with a sensitivity of 40 copies/mL. In addition, we enrolled two other groups of ART recipients using frozen sperm to compare the clinical outcomes. Results: There were five pregnancies in the fresh cycles (23.8%) of HIV-1-discordant couples and the cumulative pregnancy per couple was 42.9% (6/14). The data were comparable with normal controls and testicular sperm extraction/microscopic epididymal sperm aspiration groups. The nine babies and the 14 women in this study showed no seroconversion. Conclusion: The preliminary data showed good ART results in HIV-1-discordant couples. Fertility services should not be withheld from individuals with HIV-1, although larger series are needed to reach conclusions about safety. ? 2011 Elsevier & Formosan Medical Association.
SDGs
Other Subjects
virus RNA; adult; article; assisted reproductive technique; clinical article; controlled study; density gradient centrifugation; female; human; Human immunodeficiency virus 1 infection; infertility therapy; intermethod comparison; male; microscopic epididymal sperm aspiration; outcome assessment; pregnancy; real time polymerase chain reaction; reverse transcription polymerase chain reaction; sensitivity analysis; seroconversion; sperm retrieval; testicular sperm extraction; thawed washed sperm; virus load
Type
journal article