Use of Piezoresistive Microcantilever Biosensor to Real-Time Monitor Bio-molecules
Date Issued
2006
Date
2006
Author(s)
Yu, Yuh-Yan
DOI
zh-TW
Abstract
Bio-sensing tools have been moving towards miniaturization, high sensitivity, portability and wireless networking. While fluorescent labeling of nucleic acids is becoming a standard procedure, protein labeling techniques are not yet as well established. The purpose of this paper is to demonstrate a novel reusable biosensor which achieves the continuous label-free recognition of biological substances in real-time, which based on the nanomechanics of a piezoresistive microcantilever.
Over the past few years, an increasingly evident number of studies have been conducted on using the microcantilevers as transducers in biochemical-sensing systems. In principle, adsorption of biochemical species on a functionalized surface of a micro-cantilever will cause bio-induced surface stress and accordingly the cantilever bends. We used this technique in the immunoassay.
A microcantilever based biosensor with piezoresistive has been developed using surface micromachining technique in this paper, which is cost effective, miniaturized and high applicability. This provides a novel method to detect bio-molecules which is due to molecule binding induced cantilever deflection. We have demonstration of wireless real-time label-free detection of bio-linker and anti-CRP antibody, the signal was successfully detected by using piezoresistive cantilever biosensor. The tendency of cantilever deflection is agreement with optical-based cantilever detection. In the future, the piezoresistive cantilever biosensor will offer a powerful plateform for high-throughput bioassays in proteomics and immunology.
Subjects
壓阻式微懸臂梁感測器
惠斯登電橋電路
系統晶片
Piezoresistive
surface micromachining
Biosensor
Type
thesis
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