FIN219與FHY1基因之間調控關係之研究
Investigation of the Functional Relationships between FIN219 and FHY1 Genes.
Date Issued
2005
Date
2005
Author(s)
Jang, Duan-Ya
DOI
zh-TW
Abstract
Phytochromes are plant photoreceptors that regulate plant growth and development in response to the light environment. In Arabidopsis, phytochrome A (phyA) is the primary photoreceptor responsible for perceiving and mediating various responses to far-red light. Previous studies have identified multiple phyA signaling components, such as FIN219 (far-red insensitive 219) and FHY1 (far-red elongated hypocoty 1). FHY1 encodes a product (FHY1) that specifically transduces signals downstream of the far-red (FR) light-responsive phytochrome A (PHYA) photoreceptor. FIN219 is also involved in phytochrome A signaling pathways. Complementation test between fin219 and fhy1 show a nonallelic noncomplementation relationship, indicating possible genetic interactions between these two proteins. Hence, we carry out the yeast 2-hybrid assay to investigate this possibility. The results show that FHY1 protein can interact with FIN219 C-terminus. As well, when the FIN219 overexpression construct was introduced into the fhy1 mutant, the transgenic seedlings show shorter hypocotyls than fhy1 under FR condition, which indicates that the fhy1 mutant gains more sensitivity to the perception of FR light. Moreover, the transgenic seedlings overexpressing the FHY1 in the fin219 mutants also result in a shorter hypocotyl phenotype when compared to that of fin219. In addition, Northern analysis revealed that FHY1 transcript levels arise in fin219 mutants grown in darkness; at the same time, FIN219 transcript levels increase in fhy1 mutant as well.
In summary, the results indicate that FIN219 and FHY1 coordinate with each other by direct interaction and response to FR light.
Subjects
FIN219
FHY1基因
調控關係
Functional Relationships
FHY1
Type
other
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