The Quality Change of Adlay Bran Ethanolic Extract and its Different Fractions During Storage Period.
Date Issued
2009
Date
2009
Author(s)
Lin, Ju-Tsui
Abstract
Long-term inflammation has been proven that is related to chronic diseases such as cancer, asthma, and atherosclerosis. Adlay (Coix lachryma-jobi L var. ma-yuen Stapf) was used as food and medicine, and many researches pointed out that adlay possess immuno-mediating, hormone-regulating, anti-oxidative, anti-inflammation, and anti-cancer effects. To promote functional materials utilized comprehensively, this study supposed adlay as a nutritional supplement to evaluate the stability according to Health Food Management Law published by Department of Health. In 6 months experimental period, time points at 0, 3, and 6 month or 0, 1, 2, 3, and 6 month, the samples of ABE (adaly bran ethanolic extract) and sub-fractions (ABE-Hex, ABE-Ea, and ABE-Bu) were taken to quantify the changes of components possessed anti-inflammation activity during storage period. 8 phenolic compounds and 3 phytosterols were chosen as targets, the former comprised included chlorogenic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, 4-hydroxyacetophenone, syringaldehyde, and 6-methoxy-2-benzoxazolinine, while the latter comprised campesterol, stigmasterol, and β-sitosterol. Meantime, peroxide value (POV) was used to evaluate sample stability during storage periods. The results showed that POV in accelerating test were higher in ABE and ABE-Hex than other fractions at 0 month, and became lower as storage time passed. POV in ABE-Bu was stable and low at all time points. Phenolic compounds are abundant in ABE and sub-fractions, which located at ABE-Ea and ABE-Bu, are the most among all groups. As storage time passed, phenolic compounds contents changed in all fractions, and after 6 month all of the phenolic compounds significantly decreased. Values in phytosterols pointed out that β-sitosterol occupied the most quantity in all fractions, while ABE and ABE-Hex contained most phytosterols. After storage periods phytosterol contents decreased and at the time point 6 month the contents lowered significantly. Above all, the active compounds in ABE and fractions changed as storage time passed by, so the storage environment shall be noticed that to ensure quality of extracts. Still, qualified and quantified compounds in this study are active compounds that possess anti-inflammatory effect and ferulic acid may be mainly compound in ABE-Ea subfractions contributed to anti-inflammation.
Subjects
adlay seed
anti-inflammation
phenolic compounds
phytosterol compounds
SDGs
Type
thesis
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