Influence of Nanoscale Grooved Surface on Osteoblast-like Cell Morphology
Date Issued
2006
Date
2006
Author(s)
Ting, Yen-Chung
DOI
zh-TW
Abstract
Cells are known to respond to topographic cues in their natural nanometrical environment. The aim of this study is to investigate the influences of surface nanotopography on cell adhesion and orientation. Osteoblast-like cells (MG63) were cultured on a series of nanogrooved silicon wafer (90, 150, 250, 340 and 500 nm in width). At specific time point, the images of cells on different surfaces were captured by scanning electron microscopy, and cell morphology was analyzed by image software.
Initially, cells adhering on surfaces with wider grooves showed more elongated shape. The proportion of cells which aligned parallel to grooves was higher on surfaces with wider grooves. Surfaces with wider grooves promote cells to appear quicker respond of elongation and alignment. F-actin and nuclei aligned with grooves on patterned surfaces. The elongation of nuclei on patterned surfaces was also observed.
Besides, osteoblast-like cells were cultured on grooved surfaces pre-adsorbed with Fibronectin (Fn). Compared to surfaces without Fn preadsorption, cells displayed larger spreading area, less elongated appearance and less isotropic alignment on grooved surfaces with Fn pre-adsorption. The proportion of aligned nuclei also decreased on surfaces with Fn preadsorption. Cell morphology could be affected enormously by nano-grooves even if grooves were coated densely with adhesion-associated protein, Fn.
Subjects
骨母細胞
奈米
直溝
地形
形態
osteoblast
nanogroove
morphology
topography
Type
thesis
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