Analysis of Drosophila decapping protein 2, dDcp2, in axis determination during Drosophila oogenesis
Date Issued
2006
Date
2006
Author(s)
Chen, Chao-Han
DOI
en-US
Abstract
Decapping complex in yeast and human systems are well studied. It is reported that Dcp1 and Dcp2 form a heterodimer with decapping activity. Dcp2 is the catalytic center of decapping process and Dcp1 is believed to promote the decapping activity. And increasing lines of evidence show that many proteins may be associated in the decapping complex. Another distinct feature of Dcp1 and Dcp2 is their colocalization within cytoplasmic foci associated with other mRNA degradation components such as Xrn1, Edc3 and Dhh1. This specialized structure is called processing body (P body) which is referred to as the sites for mRNA degradation.
Our previous research uncovered Drosophila decapping protein 1,dDcp1, is a novel component of oskar mRNP complex and directs its posterior localization in the oocyte. (Lin et al., 2006b) dDcp1 mutant causes posterior group phenotype. And dDcp1 is also required for the proper posterior localization of other oskar mRNP complex component, such as Exu, Yps, and Orb. This discovery reveals the possibility of close linkage among transportation, transcription, translation and degradation.
According to the Drosophila genome wide gene BLAST result, we uncovered the putative and unique dDcp2 gene, CG6169. We analyzed the dDcp2 mutant allele, BG1766, and dDcp2 null allele, dDcp2de21. Both mutant alleles cause anterior- posterior and dorsal-ventral patterning defect. And the distinct phenotype could be the consequence of mislocalization of Osk, Stau and Vasa proteins.
The other interesting finding is that deletion of dDcp2 gene causes the enlargement and accumulation of P body-like structure, which may be the result of the mRNA decay pathway deficiency.
Subjects
果蠅去頭蓋蛋白
果蠅卵發育
dDcp2
Drosophila oogenesis
Type
other
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