Investigation of gene expression and signal transduction pathway of SUMO in sweet potato
Date Issued
2005
Date
2005
Author(s)
Chou, Yi-Hsiang
DOI
zh-TW
Abstract
Small ubiquitin-like modifer (SUMO) is a member of the superfamily of ubiquitin-like polypeptides that play a role in post-translational modification, but its functions are different from those of ubiquitin. SUMOs attach target proteins by covalent bond, and alter their function and location. The regulation of SUMO2 expression in sweet potato (Ipomoea batatas) was studied here. We had isolated a nitric oxide (NO)-induced IbSUMO2 protein from two-dimensional electrophoresis system, and also observed a significant increasing of IbSUMO2 mRNA under the treatment of NO or H2O2 induced by mechanical wounding. The increasing of IbSUMO2 protein under NO treatment was also observed by western blotting analysis. On the contrary, the application of NO or H2O2 inhibitors can reduce the expression of IbSUMO2 mRNA induced by mechanical wounding, suggesting IbSUMO2 protein induced by mechanical wounding through NO and H2O2 signal pathway. We also detected the level of IbSUMO2 conjugation under mechanical wounding and NO treatment. The wound-induced conjugate accumulation could be detected within 10 minutes, suggesting that IbSUMO2 conjugation is one of the early events for plant to response to mechanical wounding. On the other hand, immunoprecipitation of IbSUMO2 conjugated proteins were performed with SUMO antibodies, and two major bands around 45 KDa were observed. Furthermore, in transient expression system, we confirm the localization of IbSUMO2 protein within cell under NO stimulations. Conclusively, IbSUMO2 protein conjugated to its target proteins has multiple physiological functions in plant cells.
Subjects
一氧化氮
過氧化氫
訊息傳遞
Nitric oxide
hydrogen peroxide
SUMO
Type
other
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