行政院國家科學委員會專題研究計畫成果報告:以NF-kB 抑制劑及抗凝血蛇毒蛋白抑制眼球之新生血管形成(3/3)
Date Issued
2004
Date
2004
Author(s)
楊中美
DOI
922314B002161
Abstract
This study was conducted to investigate effects of pyrrolidine dithiocarbamate (PDTC), a nuclear
factor (NF)-κB inhibitor, on cytokine expressions and suppression of anterior chamber inflammation in
experimental autoimmune anterior uveitis. Uveitis was induced in the Lewis rats with the injection of
melanin-associated antigen into peritoneum and footpad. At defined time points, cytokine mRNA
expressions in the iris/ciliary body were measured by using a semiquantitative polymerase chain reaction
method. We found that interferon-γ (IFN-γ) and tumor necrosis factor (TNF)-α mRNA expression reached
peak during the active phase of uveitis, whereas interleukin (IL)-10 mRNA increased during the disease
resolution. In a separate experiment, PDTC (40mg/kg/day) was administrated intraperitoneally after
immunization. We found that PDTC effectively suppressed ocular inflammation, as indicated by reduced
clinical scores and inflammatory cells infiltration in aqueous humor and iris/ciliary body. The inhibitory
effects of PDTC are mainly resulted from inhibiting the expression of proinflammatory cytokines, TNF-α
and IFN-γ but augmenting anti-inflammatory cytokines, IL-10 expression. These findings suggest that
application of NF-κB inhibitors may be a potential therapeutic method for the treatment of acute anterior
uveitis.
Publisher
臺北市：國立臺灣大學醫學院眼科
Type
journal article
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