A human oral commensal-mediated protection against Sjögren's syndrome with maintenance of T cell immune homeostasis and improved oral microbiota.
Journal
NPJ biofilms and microbiomes
Journal Volume
11
Journal Issue
1
Pages
18
ISSN
2055-5008
Date Issued
2025-01-16
Author(s)
Tseng, Yu-Chao
Liao, Kai-Sheng
Lin, Wei-Ting
Li, Chin
Chang, Chia-Bin
Hsu, Jie-Wei
Chan, Chin-Pui
Chen, Chun-Ming
Wang, Hon-Pin
Chien, Hsiu-Chuan
Wu, Shu-Fen
Abstract
Sjögren's syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women's health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenzae in SS. Reduced salivary H. parainfluenzae levels in SS patients were confirmed through quantitative PCR. Oral H. parainfluenzae inoculation in NOD mice alleviated focal sialadenitis, improved salivary function, and reduced IFN-γCD3 and IFN-γCD8 T cells in salivary gland-draining lymph nodes, maintaining immune homeostasis against a biased type 1 response. Inoculation also enhanced salivary microbiota diversity, balanced the Firmicutes-to-Proteobacteria ratio, and reduced the overwhelming presence of Pseudomonas mendocina. In vitro, H. parainfluenzae-preconditioned A253 cells limited CD8 T cell expansion with reduced IFN-γ production. These findings suggest that H. parainfluenzae improves oral microbial diversity, promotes homeostatic T-cell immunity, and protects against SS, supporting its potential as a next-generation probiotic.
SDGs
Type
journal article