Partition Coefficient of Perfluorinated Chemical in Rats
Date Issued
2015
Date
2015
Author(s)
Chien, Ming-Yen
Abstract
The widespread environmental distribution of Perflourinated chemicals (PFCs) has been considered as a public health issue for a long time. Most of the current reserch focus on perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) compare to other PFCs. In order to know the metabolism progress of the chemical in biota. Physiologically based pharmacokinetic model (PBPK model) is a well-known method. Among numerous parameters, the paeririon coefficient (PC) for each of the organ in biota is indispensable. The male SD rats(N=40) were treated with a single dose of PFBA、PFBS、PFPeA、PFHxA、PFHxS、PFHpA by oral gavage at 500μg/kg. Subsequently, the SD rats were classified into exposure group (N=3) and control group (N=1) in each of the sacrified time point (2, 4, 8, 12, 24, 48, 96, 144, 196, 240 hour). Serum, liver, kidney, small intestine, epididymis fat were collected. After pre-treatment, the samples were analyzed for these PFCs via liquid chromatograph tandem mass spectrometer (LC-MS/MS). The aims of this study are finding out how the influences for partition coefficient of different carbon chain length and different fuction groups in male SD rats. After the gavage exposure, results shows that apart form PFHxS, most of the PFCs were quickly absorb from small intestine into blood circulation.Then distribute into other organs, at last exceret out of the body via kidney in 48 hours. On the other hand, PFHxS keep exist in biota after 240 hours. PFCs with carboxyl group (-COOH), such as PFBA, PFPeA, PFHxA, PFHpA. The descending order of partition coefficient is kidney, liver, small intestine, epididymis fat. As the carbon chain length increase, the partition coefficient for each of the organ does not change significantly. PFCs with sulfur trioxide group (-SO3), such as PFBS and PFHxS. The descending order of partition coefficient is liver, kidney, small intestine, epididymis fat. These differences attribute to PFCs with sulfur trioxide group (-SO3) are likely combined with hepatic fatty acid-binding protein in hepatic cells. Therefore, we can estimate a higher value of partition coefficient in liver.
Subjects
Perflourinated chemical
Pharmacokinetics
Partition coefficient
SD rat
SDGs
Type
thesis
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