狼尾草染色體組分子分析及孤雌生殖性狀遺傳定位
Date Issued
1998-07-31
Date
1998-07-31
Author(s)
王裕文
DOI
872313B002027
Abstract
Napier grass is one of the major forage
crops in Taiwan. The acreage is second to
the one of pangola grass, but the yielding
ability is about twice of the one of pangola
grass. Although the nutritive value is
generally poor in napier grass, the interspecific
hybrids between napier grass and
pearl millet, which considered to have better
nutritive value, did show the ability to
combine both species’ desired characteristics.
There were several clones released from such
inter-specific crosses by the National Taiwan
Animal Science Experiment Institution since
1981. Napier grass is a sexually reproduced
perennial grass. In order to preserve the
genetic composition of a genotype, napier
grass used to be propagated through cloning
of vegetative organ, which is the main
problem in expanding its acreage. The
introduction of apomixis, which is a process
of cloning genotype through seed production,
from related species within Pennisetum
family may help the expansion of napier
grass. The benefit of apomixis in fixing
genotype is obvious, but the difficulties in
identification and the instability of
expression are also associated with apomixis.
In order to overcome these problems, the
techniques of utilizing DNA markers in
breeding apomictic species are developing.
The progress is hindered by the polyploidy
nature of the most of the apomictic species.
The purpose of this project is to
simultaneously construct molecular genetic
maps of napier grass, pearl millet and
Pennesitum squamulatum from a
apomictic/sexual segregating population of a
three-way cross between (napier grass x pearl millet) x Pennesitum squamulatum. The
conservative RFLP markers will be chosen
from related species with well established
RFLP map and used in constructing the
molecular genetic map. The principle of
comparative mapping will be served as a
guide line in choosing conservative markers,
it will shorten the time for constructing map
by reducing the time needed for surveying
DNA probes. With the construction of
molecular genetic map, the apomixis trait
will be expected to be mapped.
Subjects
Forage crop
napier grass
Pennisetum purpureum L.
apomixis
genetic map
comparative mapping
polyploidy
breeding
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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