The Investigation on the Interaction among Anti-tumor Compounds derived from Adlay Bran against Human Lung and Colorectal Cancer Cells using Combination Index Methodology
Date Issued
2007
Date
2007
Author(s)
Huang, Hsuanfei
DOI
en-US
Abstract
The malignant tumor has continuously occupied the first place of the top ten leading causes of death for more than twenty years in Taiwan. Among those malignant neoplasm cases, the lung cancer and colorectal cancer are the first and third places of the top ten leading cancer, respectively. In this study, the combination index methodology was used to discuss the synergism or antagonism among these anti-tumor components from adlay bran. The result showed that caffeic acid (PA4) and 3-O-coumaroyl-β-sitosterol (PE1) appeared to be most synergistic compounds for combining with other compounds and chlorogenic acid (PA6) was the most antagonistic compound with other compounds on the growth inhibition of A549, a human lung cancer cell line; while any 2-compound combinations of palmitic acid (FA), 5-hydroxy-7-methoxy- 4’-acetylisoflavone (IF) and stigmasterol (S1) appeared to be synergistic on the growth inhibition HT-29, a human colorectal cancer cell line. Moreover, the cytotoxicity of compounds varied depending on the cell line tested and that such variation may in turn influence those effects of compound combinations. For example, in 2-compound combinations, PA4 exerted synergism when combining with other compounds to inhibit the growth of A549; while it became an antagonist toward other compounds, such as IF and FA, to inhibit the growth of HT-29. Moreover, in 3-compound combinations, the most synergistic 3-compound groups were PA4-PE1-IF and PA6-PE1-FA against A549 growth; while the most synergistic group was S1-IF-FA against HT-29 growth. On the other hand, the combination of PA4, PA6, and IF was the most antagonism on the growth inhibition of A549 and HT-29. From both cell lines, the results of the 3-compound combinations could be explained by the results of the 2-compound combinations. Last, the design of anti-cancer adlay supplement must be tumor-specific since that the cytotoxicity of the compounds varies depending on the cell line tested and that such variation may in turn influence those combined effects of the pure compounds.
Subjects
組合指數
相乘作用
拮抗作用
中間效應方程式
薏苡麩皮
Adlay bran
combination index
synergism
antagonism
the median-effect principle
SDGs
Type
thesis
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