Effect of mitomycin-C on human retinal pigment epithelium in culture
Journal
Current Eye Research
Journal Volume
16
Journal Issue
6
Date Issued
1997-07-08
Author(s)
Abstract
Purpose. To determine the effect of mitomycin-C on confluent and non-confluent human retinal pigment epithelium (RPE) in tissue culture. Methods. The effect of mitomycin-C on confluent RPE was determined by treating first passage confluent cells with 0.01, 0.1, 1, 10, 100 or 1000 micromolar (μM) mitomycin-C for 1, 3, or 7 days. The cell viablility after treatment was determined by using an esterase stain. The effect of mitomycin-C on proliferating RPE was determined by incubating non-confluent cells with the above concentrations of mitomycin-C for 20 min, 1 hour or 24 hours. Results. Mitomycin-C can be toxic to a confluent RPE monolayer, and the LD50 is 421, 28.8 or 0.0632 μM when cells are continually exposed to mitomycin-C for 1, 3 or 7 days, respectively. Exposure to mitomycin-C at concentrations ≤ 10 μM for 20-60 min significantly inhibits proliferation of non-confluent RPE. A 24 hour exposure of RPE to 1 μM mitomycin-C markedly inhibits proliferation of non-confluent RPE with minimal toxicity to confluent RPE. Conclusions. Since exposure of human RPE to mitomycin-C for 24 hours can inhibit cell proliferation at concentrations which are well-tolerated by confluent RPE, mitomycin-C may be a suitable agent for inhibiting RPE proliferation in vivo.
Subjects
Cell proliferation | Human retinal pigment epithelium (HRPE) | Mitomycin-C | Proliferative vitreoretinopathy
SDGs
Type
journal article