Rapid identification of the north-western Pacific billfish species using quantitative real-time polymerase chain reaction techniques
Journal
Marine Biology Research
Date Issued
2017-09
Author(s)
Abstract
Billfishes are important fishery resources traded and consumed worldwide. As morphological traits are usually removed during processing, molecular methods are applied to identify billfish products. In this study, the approaches of quantitative real-time PCR were developed to identify the six billfish species (Istiompax indica, Istiophorus platypterus, Kajikia audax, Makaira nigricans, Tetrapturus angustirostris and Xiphias gladius) widely distributed in the north-western Pacific Ocean. The developed singleplex systems showed high fidelities to each of the six species via either examining the ΔCt values or melting curve patterns. For samples containing multiple species, individual species are identifiable by a quantitative real-time PCR assay that includes all the singleplex systems. A multiplex system was also developed to identify unknown samples composed of a single species. The methods developed in this study provide a fast and high-throughput manner to identify the north-western Pacific billfish species when morphological traits are unavailable, such as in processed products. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Subjects
Billfish identification; food forensic; melting curve analysis; quantitative real-time PCR
SDGs
Other Subjects
bioassay; fish; geographical distribution; identification method; molecular analysis; morphology; polymerase chain reaction; quantitative analysis; real time; sampling; Pacific Ocean; Pacific Ocean (Northwest); Istiophorus platypterus; Makaira nigricans; Tetrapturus angustirostris; Xiphias gladius; Xiphiidae
Type
journal article