Comparison of DNA stabilizers and storage conditions on preserving fecal microbiota profiles
Journal
Journal of the Formosan Medical Association = Taiwan yi zhi
Journal Volume
119
Journal Issue
12
Pages
1791
Date Issued
2020-12
Author(s)
Chang, Chih-Min
Panyod, Suraphan
Abstract
Appropriate storage of fecal samples is a critical step for the unbiased analysis of microbial communities in metagenomic studies. Rapid freezing at -80 °C is usually considered to be best practice, but this approach is challenging. DNA stabilizing kits may provide a more convenient method to preserve and store clinical samples. We evaluated the reliability of two collection kits (Stratec stool collection tube with stabilizer, #1038111200 and OMNIgene.GUT OMR-200) on preserving fecal microbiota.
Subjects
16S rRNA amplicon sequencing; DNA stabilizers; Fecal microbiota; OMNIgene.GUT; Storage condition; Stratec
Publisher
ELSEVIER TAIWAN
Type
journal article